Journal: bioRxiv

Article Title: CaMKII-RNMT axis directs activity-dependent control of RNA dynamics in neurons

doi: 10.1101/2025.10.30.685591

Figure Lengend Snippet: A) Immunoflourecence (IF) staining in adult mouse brain sections using polyclonal anti-mouse RNMT raised in rabbit. RNMT (Green), DAPI (blue), MAP2 (red). (B) RNMT IF using polyclonal anti-human RNMT raised in sheep and GFP-RNMT localisation in undifferentiated hiPSC, GFP (green), RNMT (red), DAPI (blue).

Article Snippet: Immunoprecipitations were carried out overnight at 4 °C using 1 μg of polyclonal anti-GFP (Bio-Rad) antibody and 10 μl of protein G Dynabeads (Thermofisher Scientific).

Techniques: Staining

Journal: bioRxiv

Article Title: CaMKII-RNMT axis directs activity-dependent control of RNA dynamics in neurons

doi: 10.1101/2025.10.30.685591

Figure Lengend Snippet: (A) Endogenous RNMT and GFP-RNMT localisation in hiPSC-derived Dopaminergic (DA) neurons. GFP (green), RNMT staining using polyclonal anti-human RNMT raised in sheep (red), DAPI (blue). B) Primary murine neurons transfected with GFP-RNMT visualised at 7 days in vitro (DIV). GFP-RNMT expression visualised directly by fluorescence microscopy. C) RNMT in Synapsin I-positive neurites and MAP2-positive dendrites in DA neurons. RNMT staining using polyclonal anti-human RNMT raised in sheep (green), MAP2 (red), Synapsin I (red), DAPI (blue).

Article Snippet: Immunoprecipitations were carried out overnight at 4 °C using 1 μg of polyclonal anti-GFP (Bio-Rad) antibody and 10 μl of protein G Dynabeads (Thermofisher Scientific).

Techniques: Derivative Assay, Staining, Transfection, In Vitro, Expressing, Fluorescence, Microscopy